MDM2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Product Name
MDM2 Polyclonal Antibody
Catalog Number
RD71433A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Background
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
Immunogen Information
Immunogen
Synthesized peptide derived from human MDM2 around the non-phosphorylation site of Ser166.
Swissprot
Q00987
Synonyms
ACTFSDouble minute 2 proteinE3 ubiquitin-protein ligase Mdm2Hdm 2Hdm2HDMXMDM 2MDM2MDM2 oncogene E3 ubiquitin protein ligaseMdm2 p53 E3 ubiquitin protein ligase homologMdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding
Calculated MW
55 kDa
Observed MW
90 kDa
Applications
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas