His-Tag Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

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Overview

Circle
Product Name
His-Tag Monoclonal Antibody
Catalog Number
RD86705A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG1
Host

Background

Circle
The 6xHis tag, also known as polyhistidine tag, His6 tag and/or hexa histidine tag, is an amino acid motif consisting of at least 6 histidine residues fused to the carboxyl (C-) or amino (N-) terminus of a target protein in transfected cells. It is one of the simplest and most widely used purification tags in the world.

Immunogen Information

Circle
Immunogen
Synthetic peptide corresponding to 6His tag conjugated to keyhole limpet haemocyanin.
Synonyms
6 His epitope tagHexa His tagHHHHHH epitope tagHHHHHH tagHis tagPolyhistidine Tag
Calculated MW
40kDa
Observed MW
40kDa

Applications

Circle
Reactivity
All
Tested Applications
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IP 3ug/sample,FCM:0.2 ug
Concentration
1mg/mL
Storage Buffer
PBS with 0.1% sodium azide, 50% glycerol, pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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