Flag-Tag Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

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Overview

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Product Name
Flag-Tag Monoclonal Antibody
Catalog Number
RD86725A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG2a
Host

Background

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FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This antibody detects FLAG tag in most applications.

Immunogen Information

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Immunogen
Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin.
Synonyms
DDDDK epitope tagDDDDK epitope tagDYKDDDDK epitope tag

Applications

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Reactivity
All
Tested Applications
Conjugation
Unconjugated
Dilution
WB 1:5000-1:10000, IF 1:500-1:2000,IP 3ug/sample,FCM:0.2 ug
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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